Transcriptome survey for Helicoverpa armigera Chemosensory genes (BGI... - SRA

SRX518085: Transcriptome survey for Helicoverpa armigera Chemosensory genes (BGI data): Female-adult-tarsus
1 ILLUMINA (Illumina HiSeq 2000) run: 23.2M spots, 4.6G bases, 2.9Gb downloads

Design: Approximately 50 adult tarsi mouthparts were dissected from female adults and stored immediately in RNAlater. Total RNA was purified using RNeasy (Qiagen, USA) or RNAqueous (Ambion, USA) kits according to the manufacturer's protocol. The purified RNA was then treated with DNaseI (Ambion, USA) at 37 degrees C for 30 min along with the manufacturer's protocol, quantified and qualified by NanoDrop ND-2000 (Thermo Scientific, USA) as well as 2100 Bioanalyzer (Agilent, USA). The cDNA templates were prepared from purified RNA samples using SuperScript III Reverse Transcriptase (Invitrogen, USA), according to the manufacturer's manual. Briefly, the first-strand cDNA was synthesized with Oligo(dT)20 primer at 65 degrees C for 5 min, and then at 50 degrees C for 60 min. The reaction was stopped at 70 degrees C for 15 min. The templates were stored at -20 degrees C until use. Sequencing was performed at BGI-Tech with Illumina HiSeq 2000.

Submitted by: CSIRO

Study: Helicoverpa armigera strain:GR Transcriptome or Gene expression

PRJNA244590 • SRP041166 • All experiments • All runs

show Abstracthide Abstract

Transcriptome survey for identifying genes relevant to chemoreception.

Sample: Helicoverpa armigera female adult tarsi library

SAMN02727612 • SRS592813 • All experiments • All runs

Organism: Helicoverpa armigera

Library:

Name: Female-adult-tarsus

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq