GSM3560793: Unf3; Anopheles aquasalis; RNA-Seq - SRA

SRX5243600: GSM3560793: Unf3; Anopheles aquasalis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 25.5M spots, 5.1G bases, 2Gb downloads

Submitted by: NCBI (GEO)

Study: De novo transcriptome sequencing in Anopheles aquasalis using RNA-seq

PRJNA514802 • SRP178562 • All experiments • All runs

show Abstracthide Abstract

Anopheles aquasalis transcriptome in response to Plasmodium vivax invasion of midgut Overall design: A. aquasalis mosquitoes were: i) membrane-fed on P. vivax infected blood, ii) membrane-fed on blood with inactivated gametocytes or iii) fed on 10 % sugar solution. 18-24 hours after feeding, mosquitoes were dissected and pools of 30 midguts were collected from each group. Three independent biological replicates of each experiment were performed.

Sample: Unf3

SAMN10734507 • SRS4246265 • All experiments • All runs

Organism: Anopheles aquasalis

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Mosquito midguts were collected from mosquitoes submerged in ice-cold PBS and transferred to RNAlater (Ambion). RNA was harvested using Trizol reagent and treated with RNase-Free DNase I (Qiagen). RNA integrity was confirmed using 2100 Bioanalyzer (Agilent) and total RNA was used for library preparation and sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM3560793

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 25.5M spots, 5.1G bases, 2Gb

Run	# of Spots	# of Bases	Size	Published
SRR8457192	25,457,565	5.1G	2Gb	2019-06-11

ID:: 7079666

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