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SRX641442: Arachis hypogaea transcriptional gene finding- Pdui1
1 LS454 (454 GS FLX Titanium) run: 168,728 spots, 85.9M bases, 193.1Mb downloads

Design: To isolate genome-wide expression genes from peanut, a high yield and quality peanut cultivar (Arachis hypogaea cv Minhua6) was used for gene discovery. Eight tissues of root, stem, leaf, flower, peg, pod perecarp, testa and embryo of different developing stages and yong seedlings were used for RNA extraction and the equally mixed RNA was used to prepare a whole tissues cDNA library. Additionally, samples from treatments of biotic and abiotic stresses including low temperature and drought, low Ca2+ stresses, hormone treatments (ethylene, abscisic acid, salicylic acid and paclobutracol), and Aspergillus flavus, Rolstonia solanacearum inoculation carried out in peanut, and their corresponding controls were taken for RNA extraction, respectively. Then equally mixed stressed RNAs and mixed control RNAs were used to prepare two cDNA libraries, a stressed cDNA and a control cDNA pools. Then, cDNA libraries were subjected to 454 GS FLX Titanium Pyrosequencing, respectively.
Submitted by: Fujian Agriculture and Forestry University
Study: Arachis hypogaea strain:Minhua6 Transcriptome or Gene expression
show Abstracthide Abstract
To isolate genome-wide expression genes from peanut, a high yield and quality peanut cultivar (Arachis hypogaea cv Minhua6) was used for gene discovery. Eight tissues from root, stem, leaf, flower, peg, pod perecarp, testa and embryo of different developing stages and young seedlings were used for RNA extraction. Equally mixed RNA was used to prepare cDNA library. Additionally, samples subjected to biotic and abiotic stress, which included low temperature and drought, low Ca2+, hormone treatments (ethylene, abscisic acid, salicylic acid and paclobutracol), and inoculation with Aspergillus flavus and Rolstonia solanacearum and their corresponding controls were taken for RNA extraction. Then equally mixed stressed RNAs and mixed control RNAs were used to prepare two cDNA libraries, a stressed cDNA and a control cDNA library. cDNA libraries were then subjected to 454 XLR70 Titanium Pyrosequencing, respectively.</Description> 10 Additionally, samples subjected to biotic and abiotic stress, which included low temperature and drought, low Ca2 , hormone treatments (ethylene, abscisic acid, salicylic acid and paclobutracol), and inoculation with Aspergillus flavus and Rolstonia solanacearum and their corresponding controls were taken for RNA extraction. Then equally mixed stressed RNAs and mixed control RNAs were used to prepare two cDNA libraries, a stressed cDNA and a control cDNA library. cDNA libraries were then subjected to 454 XLR70 Titanium
Sample: To isolate genome-wide expression genes from peanut, a high yield and quality peanut cultivar (Arachis hypogaea cv Minhua6) was used for gene discovery. Eight tissues of root, stem, leaf, flower, peg, pod perecarp, testa and embryo of different developing stages and yang seedlings were used for RNA extraction and the equally mixed RNA was used to prepare a whole tissues cDNA library.
SAMN02837507 • SRS629957 • All experiments • All runs
Library:
Name: Peanut whole tissue library
Instrument: 454 GS FLX Titanium
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: SINGLE
Spot descriptor:
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Runs: 1 run, 168,728 spots, 85.9M bases, 193.1Mb
Run# of Spots# of BasesSizePublished
SRR1368960168,72885.9M193.1Mb2014-06-30

ID:
897401

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