RNA-sequencing of Drosophila simulans adult female brain - SRA

SRX6438792: RNA-sequencing of Drosophila simulans adult female brain
1 ILLUMINA (NextSeq 500) run: 33M spots, 9.9G bases, 3.9Gb downloads

Design: Prior to the dissections, all fly lines were expanded and maintained at 24C under an undisturbed 12:12 light/dark cycle in low density vials with fly food. All individuals were mated and precisely at 4-days-old. Flies were very briefly anesthetized with CO2 and heads were collected with a clean razor blade. Heads were transferred to a glass dissection plate with PBS for brain collection. Dissections were performed within a 3-hour window always at the same circadian zeitgeber time (ZT1-ZT4; in this experiment the light was turned on at 8 am, and the dissections were performed between 9 am and 12 pm within a three-hour interval). Male and female brains of the same line were dissected in parallel the same day at the same time. Dissected brains were immediately transferred into a low retention Eppendorf tube containing 100 mL Trizol (Invitrogen) and kept frozen at -20C until RNA extraction. We collected 3 biological replicates for each genotype by sex combination, and each replicate comprised of a pool of exactly 15 brains.

Submitted by: The Rockefeller University

Study: Transcriptomes of the brain of Drosophila species subgroup

PRJNA553234 • SRP214528 • All experiments • All runs

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In this study, we generated and analyzed population scale gene expression data from female and male brains of several strains of Drosophila melanogaster, D. simulans, and D. yakuba to answer questions on gene expression evolution, gene origination, and genome evolution.

Sample: w501_female_brain_rep1_L1

SAMN12261919 • SRS5093456 • All experiments • All runs

Organism: Drosophila simulans

Library:

Name: w501_female_brain_rep1_L1

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 33M spots, 9.9G bases, 3.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR9678417	33,006,298	9.9G	3.9Gb	2020-06-10

ID:: 8531063

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