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SRX6595837: RNA-seq of Oncorhynchus gorbuscha: Pink salmon white muscle
1 ILLUMINA (Illumina NovaSeq 6000) run: 50.8M spots, 15.3G bases, 4.5Gb downloads

Design: RNA was extracted from tissue using the Qiagen RNeasy kit, quantified by Bioanalyzer and shipped to the McGill University and Genome Quebec Innovation Centre. mRNASeq stranded libraries were built, with NEBNext dual index adapters. Library was sequenced as a 1/39 fraction of a NovaSeq 6000 S4 PE150 lane.
Submitted by: Fisheries and Oceans Canada
Study: Genome assembly of Pink salmon (Oncorhynchus gorbuscha)
show Abstracthide Abstract
Pink salmon (Oncorhynchus gorbuscha) is a species of anadromous salmon native to streams and rivers of the northern Pacific Ocean. They are often referred to by the common name humpbacks due to the large hump that appears behind the head in sexually mature males. Populations are separated into even and odd year classes, as the strict two-year life cycle means returning year classes never mix. Self-sustaining populations have been ascribed primarily from Puget sound in Washington State up through to the MacKenzie River, and from the Japanese island of Hokkaido through to the Lena River in the Arctic Ocean (Behnke 2002) – however, pink salmon are noted for straying resulting in populations described as far south as Central California, and moving deeper into the Arctic Ocean and up the MacKenzie River – recent reports of potential straying include to Newfoundland, Greenland, Norway and northern Europe. This work aims to establish genomic baseline for the species by assembling a reference genome from an odd-year pink salmon haploid. Gene annotation is provided by Illumina RNA-seq across 19 distinct tissues. Resequencing of pink salmon, sampled range-wide and across both year-classes, aims to categorize geographic and year-class defining variation genome-wide.
Sample: Pink White Muscle
SAMN12367913 • SRS5161191 • All experiments • All runs
Library:
Name: Pink-White_Muscle
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Runs: 1 run, 50.8M spots, 15.3G bases, 4.5Gb
Run# of Spots# of BasesSizePublished
SRR984110350,827,11315.3G4.5Gb2020-08-21

ID:
8701896

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