Roots infected by Verticillium dahliae 48h Olea europaea - SRA

SRX661365: Roots infected by Verticillium dahliae 48h Olea europaea
2 ILLUMINA (Illumina HiSeq 1000) runs: 54.4M spots, 11G bases, 7.1Gb downloads

Design: RNA was extracted from roots of three plants inoculated with Verticillium dahliae 48h before. Total RNA was extracted using a Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions. Any DNA contamination was removed by DNaseI treatment on column (Roche, Basel, Switzerland). The RNA-quality tests were performed with the Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) using an RNA 6000 Pico assay kit (Agilent Technologies, Santa Clara, CA, USA). We pooled RNA and made cDNA libraries for sequencing in Illumina HiSeq 1000 sequencer. Two replicates were sequenced on different lines. After trimming and cleaning, we obtained 54.358.741 paired-end reads of 101bp length and 35.4 Phred quality (11 Gb).

Submitted by: Universidad de Jaen

Study: Olea europaea Transcriptome or Gene expression

PRJNA256033 • SRP044780 • All experiments • All runs

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Control and plants responding to biotic and abiotic stress were sequenced by Illumina HiSeq 1000 paired-end.

Sample:

SAMN02937431 • SRS666308 • All experiments • All runs

Organism: Olea europaea

Library:

Name: Roots Vd 48h

Instrument: Illumina HiSeq 1000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Spot descriptor:

1 forward102 reverse

Runs: 2 runs, 54.4M spots, 11G bases, 7.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR1525086	27,214,837	5.5G	3.5Gb	2014-11-14
SRR1525087	27,143,904	5.5G	3.5Gb	2014-11-14

ID:: 927877

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