GSM1477387: aFLe1_mRNAseq; Anolis carolinensis; RNA-Seq - SRA

SRX679301: GSM1477387: aFLe1_mRNAseq; Anolis carolinensis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 23.9M spots, 1.2G bases, 727.8Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: A relative shift in cloacal location repositions external genitalia in amniote evolution

PRJNA258129 • SRP045471 • All experiments • All runs

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Comparative RNA-seq profiling of mouse and anole lizard developing limbs and external genitalia, to assess evolutionary and develomental relationships, between the two tissue types based on transcriptomic data Overall design: RNA-seq profiling of embryonic limb and external genitalia tissue at different stages of development, in mouse and anole lizard, in duplicates, using Illumina HiSeq

Sample: aFLe1_mRNAseq

SAMN02988838 • SRS682458 • All experiments • All runs

Organism: Anolis carolinensis

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted using Arcturus PicoPure RNA Isolation Kit (Life Technologies) and enriched for mRNA fraction using MPG mRNA Purification Kit (PureBiotech) Multiplexed RNA-seq libraries were produced using the SPIA cDNA synthesis kit and the Ovation Ultralow DR Multiplex system (NuGen)

Experiment attributes:

GEO Accession: GSM1477387

Links:

External link: GEO Sample GSM1477387

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 23.9M spots, 1.2G bases, 727.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR1548569	23,947,799	1.2G	727.8Mb	2014-08-15

ID:: 951311

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Sequence Read Archive

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