Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Sequencing libraries were prepared from either 100 ng or one µg of total RNA using the Illumina TruSeq RNA Sample preparation Kit v2 according to manufacturer's instructions. Sequencing libraries were quantified using the Kapa Library Quantification kit (Kapa Biosystems) and quality controlled by capillary electrophoresis on a Bioanalyzer using DNA 1000 chips (Agilent Technologies). Libraries were sequenced on a HiSeq2500 sequencer (Illumina) for 2 x 125 cycles using version 3 cluster generation kits and version 3 sequencing reagents (Illumina). The PhiX control library (Illumina) was spiked into each sample (at 1%) as a sequencing control.