Control of larvae Songpu mirror carp biological replicate #1 - SRA

SRX6908946: Control of larvae Songpu mirror carp biological replicate #1
1 ILLUMINA (NextSeq 500) run: 12.6M spots, 2G bases, 937.3Mb downloads

Design: One microgram of total RNA of each sample was used as the initial material for library construction. DNase I (RNase free) from Promega was used to eliminate genomic DNA contamination and 1 g DNase I-treated total RNA of each sample was used as the beginning material. The NEBNext rRNA Depletion Kit was used for rRNA depletion. After that, the RNAs were purified by using the Agencourt RNAClean XP Beads from Beckman Coulter. Subsequently, RNA fragmentation, first strand and second strand cDNA synthesis and double-stranded cDNA end repair were performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of the NEBNext Multiplex Oligos for Illumina. Finally, the Q5 Hot Start HiFi PCR Master Mix (NEB) was used for PCR enrichment of the adaptor-ligated DNA. Concentration and quality of the libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies.

Submitted by: Chinese Academy of Sciences

Study: Transcriptome of larvae Songpu mirror carp and Barbless carp

PRJNA573989 • SRP223359 • All experiments • All runs

show Abstracthide Abstract

Songpu mirror carp and Barbless carp are two closely related species of the carp family and they can hybrid to generate the offspring. In addition, Songpu mirror carp exhibited stronger abilities of cold tolerance and acclimation than Barbless carp;then, we compared transcriptomic profiles of two carp species to identify key signaling factors and pathways for cold tolerance and acclimation.

Sample: Control of larvae Songpu mirror carp biological replicate #1

SAMN12838222 • SRS5440726 • All experiments • All runs

Organism: Cyprinus carpio

Library:

Name: Sp_Ctrl#1

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 12.6M spots, 2G bases, 937.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR10188531	12,634,686	2G	937.3Mb	2020-06-04

ID:: 9091330

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity