GSM1513946: NG_Seven_days_after_exposure_rep2; Solanum tuberosum; RNA... - SRA

SRX710701: GSM1513946: NG_Seven_days_after_exposure_rep2; Solanum tuberosum; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer II) run: 57.7M spots, 2.9G bases, 1.8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Transcriptional changes in dormant potato minitubers treated with 1-(alpha-ethylbenzy)-3-nitroquanidine

PRJNA262145 • SRP047517 • All experiments • All runs

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RNA was sequenced from commercially grown greenhouse minitubers treated with 10 ug of 1-(alpha-ethylbenzy)-3-nitroquanidine (NG) in DMSO and followed for four, and seven days post treatment. Control tubers were injected with DMSO alone. Sequence reads were mapped to the potato genome (PGSC_DM_v3_2.1.11) and transcript abundance was determined using Cuffdiff v 2.02. Overall design: Minitubers were injected with DMSO (control) or 10 ug of NG and then trancript changes were examined at on, four, and seven days after exposure.

Sample: NG_Seven_days_after_exposure_rep2

SAMN03081362 • SRS709447 • All experiments • All runs

Organism: Solanum tuberosum

Library:

Instrument: Illumina Genome Analyzer II

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: One to two mm thick disc containing the apical bud was frozen in liquid N2. RNA was isolated using an Ambion RNA extraction kit. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1513946

Links:

External link: GEO Sample GSM1513946

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 57.7M spots, 2.9G bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR1586401	57,657,436	2.9G	1.8Gb	2014-12-01

ID:: 1001895

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