RNA-seq of Dipodomys spectabilis adult male spleen: Sample Model orga... - SRA

SRX745244: RNA-seq of Dipodomys spectabilis adult male spleen: Sample Model organism or animal sample from Dipodomys spectabilis
1 ILLUMINA (Illumina HiSeq 2000) run: 6.2M spots, 1.2G bases, 721.2Mb downloads

Design: "This is paired end sequencing data of RNA extracted with TRIZOL from a sample of spleen tissue from Dipodomys spectabilis from Portal, AZ collected in December 2009. Tissue was minced and frozen in liquid nitrogen prior to transport to Purdue University for RNA extraction. The sequencing library was constructed according to Illumina methods by Purdue Genomics, barcoded, and sequenced with 23 other sequencing libraries (24 Libraries total, spleen and spleen from each of 4 individuals from 3 different species). These 24 libraries were sequenced on one lane in Fall 2012 (10_19_12 tag in file name) and all were sequenced again on one lane of an Illumina HiSeq 2000 in January 2013 (1_10_13 tag in file name). The reads here were trimmed to remove sequencing primers and poor quality bases. They were further processed and concatenated for downstream analyses and assemblies described in subsequent publications (Marra et al 2014)"

Submitted by: Purdue University

Study: Dipodomys spectabilis Transcriptome or Gene expression

PRJNA261897 • SRP049342 • All experiments • All runs

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In this project we conducted RNA seq of spleen tissue from multiple individuals of three different species of Heteromyid rodents. Two of these species, Dipodomys spectabilis and Chaetodipus baileyi, are species from temperate latitudes. The third, Heteromys desmarestianus, is a tropical species from wet habitats. The study sought to identify differences in the sequence and expression of genes expressed in spleen tissue between the temperate species and the tropical. The goal was to understand the the immunogenetic differences between taxa that from temperate and tropical latitudes while providing genetic resources for the study of immunity in the Heteromyidae.

Sample: This is data from RNA seq of Kidney and spleen tissue from one adult male Dipodomys spectabilis (banner-tailed kangaroo rat) that was collected in December 2009. Data for two studies that looked at genetic basis of efficient osmoregulation and expression of immunity related genes, respectively, in Heteromyid rodents.

SAMN02726256 • SRS701633 • All experiments • All runs

Organism: Dipodomys spectabilis

Library:

Name: E84S_10_19_12

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 6.2M spots, 1.2G bases, 721.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR1633386	6,201,792	1.2G	721.2Mb	2014-11-01

ID:: 1076552

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