GSM4275543: T7A; Nematostella vectensis; RNA-Seq - SRA

SRX7575844: GSM4275543: T7A; Nematostella vectensis; RNA-Seq
4 ILLUMINA (Illumina HiSeq 2500) runs: 4.1M spots, 410.5M bases, 127.1Mb downloads

Submitted by: NCBI (GEO)

Study: Global temporal gene expression of Nematostella vectensis in-situ

PRJNA601911 • SRP242562 • All experiments • All runs

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We assessed genome-wide temporal transcript expression patterns in the sea anemone, Nematostella vectensis, in Great Sippewissett Marsh in Massachusetts, where anemones experienced a natural light cycle with intensity varying from 0-200 lum/ft2, daily temperature fluctuations of ~9C. We measured 'in situ' gene expression from recaptured anemones every hour from 0800 to 1700 and identified six time-dependent gene clusters, represented by several genes involved in metabolism, stress, and transcription-translation related functions. Overall design: We measured gene expression from anemones every hour from 0800 to 1700 for a total of 10 time points in both single individuals and pooled samples

Sample: T7A

SAMN13876867 • SRS6010292 • All experiments • All runs

Organism: Nematostella vectensis

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Whole-organism RNA was extracted from animals (that were preserved in RNAlater and stored at -80) using Ambion RNAqueous extraction kit. Libraries were prepared as in Meyer et al. (2011)

Experiment attributes:

GEO Accession: GSM4275543

Links:

NCBI link: NCBI Entrez (gds)

Runs: 4 runs, 4.1M spots, 410.5M bases, 127.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR10907921	921,339	93.1M	29.3Mb	2020-01-20
SRR10907922	1,009,583	102M	31.5Mb	2020-01-20
SRR10907923	1,058,058	106.9M	32.9Mb	2020-01-20
SRR10907924	1,075,514	108.6M	33.4Mb	2020-01-20

ID:: 9894825

SRA

Sequence Read Archive

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