GSM4279340: CD-T4E_S185; Nematostella vectensis; RNA-Seq - SRA

SRX7619010: GSM4279340: CD-T4E_S185; Nematostella vectensis; RNA-Seq
6 ILLUMINA (Illumina HiSeq 2500) runs: 4.3M spots, 431.9M bases, 149.6Mb downloads

Submitted by: NCBI (GEO)

Study: Gene expression during recovery from different forms of trauma in the sea anemone, Nematostella vectensis

PRJNA602703 • SRP243928 • All experiments • All runs

show Abstracthide Abstract

We exposed N. vectensis to three forms of trauma (streptomycin, cytochalasin D, or calcium-free seawater) to determine differences in gene expression during recovery from the trauma. Gene expression was monitored during different time points of recovery for animals exposed to cytochalasin D or calcium-free seawater. Gene expression was monitored following designated times of exposure and after 1 hour of recovery for animals exposed to streptomycin. Overall design: total 84 samples: 14 time points (3 replicates each) for 2 conditions. (14*3*2 = 84)

Sample: CD-T4E_S185

SAMN13901811 • SRS6051389 • All experiments • All runs

Organism: Nematostella vectensis

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted from oral discs and tentacles (flash frozen in liquid nitrogen/stored at -80) using Ambion RNAqueous extraction kit. Libraries were prepared as in Meyer et al. (2011)

Experiment attributes:

GEO Accession: GSM4279340

Links:

NCBI link: NCBI Entrez (gds)

Runs: 6 runs, 4.3M spots, 431.9M bases, 149.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR10952354	784,000	79.2M	27Mb	2020-01-23
SRR10952355	677,789	68.5M	23.1Mb	2020-01-23
SRR10952356	707,632	71.5M	25.8Mb	2020-01-23
SRR10952357	714,211	72.1M	25.5Mb	2020-01-23
SRR10952358	683,357	69M	24.6Mb	2020-01-23
SRR10952359	709,125	71.6M	23.7Mb	2020-01-23

ID:: 9944669

SRA

Sequence Read Archive

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