directional RNA-Seq of Bison bison: adult female spleen: Sample B423SP - SRA

SRX765351: directional RNA-Seq of Bison bison: adult female spleen: Sample B423SP
1 ILLUMINA (Illumina HiSeq 2500) run: 33M spots, 6.6G bases, 4.2Gb downloads

Design: "Tissue was harvested immediately after euthanasia. Approximately 100 mg of tissue was flash-frozen in liquid nitrogen and stored at -80 degrees Celsius until RNA was extracted. Frozen tissue was pulverized using a mortar and pestle, and total RNA was extracted using TRIzol RNA Isolation methods (Life Technologies). Quantitative and qualitative RNA assessments were made using Nanodrop (Thermo Scientific) and Bioanalyzer 2100 (Agilent ) using the RNA 6000 Nano chip. A 2-ug total RNA sample was suspended in 10 uL of DEPC-treated water. Total RNA was depleted of rRNA and the libraries were prepared using the TruSeq stranded total RNA sample preparation kit for human/mouse/rat (Illumina). The sizes of the libraries were determined on a Bioanalyzer 2100 (Agilent) using the DNA1000 chip and quantifiedon aQubit 2.0 using the broad range DNA assay (Invitrogen). Libraries were diluted to 2 uM and pooled. The pooled libraries were quantified again, this time using the Qubit high sensitivity assay, to recheck concentrations.Prepared samples were loaded into six lanes on a high output flow cell ??at a concentration of 7 pM and cluster generation was performed usinga cBot (Illumina). The flowcell was runonan IlluminaHiSeq 2500 (2X100 High Output)."

Submitted by: (USDA_ARS_NADC)

Study: Bison bison bison isolate:TAMUID 2011002044 Genome sequencing and assembly

PRJNA257088 • SRP050120 • All experiments • All runs

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American bison Whole Genome Sequence

Sample: spleen tissue isolated for directional mRNASeq to assist in genome annotation

SAMN03201492 • SRS749653 • All experiments • All runs

Organism: Bison bison

Library:

Name: B423SP_lane2

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 33M spots, 6.6G bases, 4.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR1659068	32,993,571	6.6G	4.2Gb	2014-12-03

ID:: 1105274

SRA

Sequence Read Archive

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