Fresh 1d larvea - SRA

SRX7767477: Fresh 1d larvea
1 ILLUMINA (Illumina HiSeq 4000) run: 40.6M spots, 12.2G bases, 4.3Gb downloads

Design: The fresh 1 day larvae, as a blank control group, were hatched from fresh embryos without any treatment. RNA from three larvae of each replicate was isolated using Trizol reagent (Invitrogen, USA), with concentration and purity being determined using NanoDrop (Thermo Fisher Scientific, Massachusetts, USA). After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads, while prokaryotic mRNA was enriched by removing rRNA by Ribo-ZeroTM Magnetic Kit (Epicentre). Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers. Second-strand cDNA were synthesized by DNA polymerase I, RNase H, dNTP and buffer. Then the cDNA fragments were purified with QiaQuick PCR extraction kit, end repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina HiSeqTM 4000 by Gene Denovo Biotechnology Co. (Guangzhou, China).

Submitted by: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences

Study: Embryo-cryopreservation larvae RNA-sequencing

PRJNA607917 • SRP250258 • All experiments • All runs

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Here, we employed high throughput sequencing technology to obtain the transcriptome of kelp grouper (Epinephelus moara) from fresh larvae and hatching larvae from cryopreserved embryos.

Sample: Blank control

SAMN14151390 • SRS6184412 • All experiments • All runs

Organism: Epinephelus moara

Library:

Name: BC-3_S1_R_PE_HiSeq4000

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 40.6M spots, 12.2G bases, 4.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR11130960	40,627,269	12.2G	4.3Gb	2021-02-20

ID:: 10145596

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