liver - SRA

SRX7868979: liver
1 ILLUMINA (Illumina HiSeq 4000) run: 103.3M spots, 31G bases, 11.6Gb downloads

Design: Total RNA was extracted from RNAlater-preserved tissue (intestine, lungs, liver, muscle) of the same individual used for the genomic sequencing using PureLink RNA Mini Kits (Life Technologies, Carlsbad, CA), following an adapted protocol from Gunter et al. (2013). RNA samples were quantified with a Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA) and quality was assessed with a 2200 Tapestation (Agilent, Santa Clara, CA). The libraries were prepared with Illumina TruSeq Total Stranded RNA-seq protocol and sequenced together on one lane of Illumina HiSeq4000 (150 bp paired-ends).

Submitted by: University of Glasgow

Study: Chromosome-level assembly of the common lizard (Zootoca vivipara) genome

PRJNA610958 • SRP251946 • All experiments • All runs

show Abstracthide Abstract

Squamate reptiles exhibit high variation in their traits and geographical distribution and are therefore fascinating taxa for evolutionary and ecological research. However, high-quality genomic recourses are very limited for this group of species, which inhibits some research efforts. To address this gap, we assembled a high-quality genome of the common lizard Zootoca vivipara (Lacertidae) using a combination of high coverage Illumina (shotgun and mate-pair) and PacBio sequence data, with RNAseq data and genetic linkage maps.

Sample:

SAMN14329711 • SRS6277498 • All experiments • All runs

Organism: Zootoca vivipara

Library:

Name: AY10694EK0004

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM PCR

Layout: PAIRED

Runs: 1 run, 103.3M spots, 31G bases, 11.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR11262332	103,321,580	31G	11.6Gb	2020-03-07

ID:: 10298463

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