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SRX790855: GSM1556233: muscle28 [tilapia]; Oreochromis niloticus; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer II) run: 5.4M spots, 832.6M bases, 539.3Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Comparison of low temperature transcriptomes between tilapia and zebrafish
show Abstracthide Abstract
Changes in gene expression are thought to be required for maintaining tissue''s proper function under cold stress, but little is known about the role of each tissue in setting the cold tolerance ability in fish. Oreochromis niloticus and Danio rerio are two tropical fishes; they differ in their abilities of cold tolerance. In this study, both fishes were exposed to graded cold temperatures, ranging from 28°C to 18°C and 10°C, and the transcriptomes of 8 tissues at each tempeature were sequenced and compared. By characterizing tissue-based gene expression variation between the two fishes we identified some key tissue specific biological processes, which provide a better understating of the typical biological functions of tissues in physiological fitness under cold stress. Overall design: Examination of gene expression of 21 tilapia samples (seven tissues at three temperatures) This submission represents ''tilapia'' transcriptome component of study.
Sample: muscle28 [tilapia]
SAMN03246867 • SRS774780 • All experiments • All runs
Library:
Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Tissues were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM1556233
Links:
External link:
Runs: 1 run, 5.4M spots, 832.6M bases, 539.3Mb
Run# of Spots# of BasesSizePublished
SRR16859335,398,239832.6M539.3Mb2017-11-30

ID:
1132786

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