RNA-seq of Aplysia california: 12 months BSC - SRA

SRX8589074: RNA-seq of Aplysia california: 12 months BSC
1 ILLUMINA (Illumina HiSeq 2500) run: 17.6M spots, 3.3G bases, 2.2Gb downloads

Design: total RNA extracted with Qiagen RNEasy Micro-kit from homogenized sensory neuron clusters. Libraries generated with Illumina TruSeq mRNA Stranded HT kit. Total RNA was poly-A selected with oligo-dT beads. First strand synthesis was random primed. Libraries were multiplexed and sequenced across 8 lanes on an Illumina HiSeq 2500 as paired end 100 base pair reads.

Submitted by: University of Miami Rosenstiel School of Marine and Atmospheric Science

Study: Aplysia californica Sensory Neuron Time Series Transcriptome

PRJNA639857 • SRP268155 • All experiments • All runs

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The goal of this study is to sequence the transcriptomes of targeted sensory neuron clusters in the Aplysia californica nervous system over the course of the adult aging process to identify transcriptional events that underpin neuronal and cognitive dysfunction previously observed to occur in this model species. This is the first such time series RNAseq experiment to be done in the A. californica nervous system.

Sample:

SAMN15282853 • SRS6880542 • All experiments • All runs

Organism: Aplysia californica

Library:

Name: P17

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Runs: 1 run, 17.6M spots, 3.3G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR12061243	17,614,505	3.3G	2.2Gb	2020-08-11

ID:: 11166267

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