With the purpose of gaining some insights on coffee resistance proces... - SRA

SRX860916: With the purpose of gaining some insights on coffee resistance process, a RNA Illumina sequencing approach was used to characterize the transcriptional profile of two coffee genotypes, respectively resistant (Catimor 88) and susceptible (Caturra) to C. ka
4 ILLUMINA (Illumina HiSeq 2000) runs: 29.6M spots, 5.7G bases, 3.8Gb downloads

Design: Total RNA was isolated from hypocotyls of all samples with SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA), according to the manufacturer’s instructions. Total RNA purity and concentration was measured at 260/280 nm and 260/230 nm using a spectrophotometer (NanoDrop- 1000, Thermo Scientific), while RNA integrity was verified by gel electrophoresis.

Submitted by: CIFC-Instituto de Investigacao CientificaC

Study: Coffea arabica cultivar:Catimor and Caturra Transcriptome or Gene expression

PRJNA271934 • SRP052734 • All experiments • All runs

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Coffee berry disease (CBD), caused by the fungus Colletotrichum kahawae, is considered one of the biggest threats to Arabica coffee production, at high altitude, in Africa. Some coffee genotypes are known to be resistant to CBD, but the molecular genetic basis of coffee resistance is still unknown. The goal of this project is to analyse the expression differences between two cultivars of Coffea spp. - one resistant, other susceptible - during the early stages of infection of the pathogen Colletotrichum kahawae.

Sample: S2Q24

SAMN03279883 • SRS832507 • All experiments • All runs

Organism: Coffea arabica

Library:

Name: S2Q24

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: unspecified

Layout: PAIRED

Spot descriptor:

1 forward151 reverse

Pipeline: show...hide...

Program	Version
CASAVA	1.8

Runs: 4 runs, 29.6M spots, 5.7G bases, 3.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR1781776	7,366,529	1.4G	960.4Mb	2016-01-09
SRR1781778	7,372,286	1.4G	959Mb	2016-01-09
SRR1781782	7,351,697	1.4G	959.1Mb	2016-01-09
SRR1781784	7,548,490	1.5G	991.1Mb	2016-01-09

ID:: 1233723

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