Instrument: Illumina HiSeq 1500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was extracted using the Qiagen RNeasy Plant Kit using the RLC lysis buffer supplemented with 25 mg/mL PEG 8000 and following manufactuers instructions thereafter. RNA quantity was analyzed using the QuBit broad spectrum RNA kit as per manufacturers instructions and RNA quality was verified using the BioAnalyzer platform (Agilent Technologies Inc.). cDNA libraries were prepared for sequencing using standard Illumina protocols by the University of Western Sydney Next Generation Sequencing Facility (Richmont,Australia)