Red clover IFS1 KO expression - SRA

SRX8970453: Red clover IFS1 KO expression
1 ILLUMINA (Illumina HiSeq 2000) run: 21M spots, 6.3G bases, 1.8Gb downloads

Design: mRNA was enriched using oligo(dT) beads, rRNA removed using the Ribo-Zero kit leaving mRNA. The mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers primer, followed by second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. Following a series of terminal repair, ligation and sequencing adaptor ligation, the double-stranded cDNA library completed through size selection and PCR enrichment.

Submitted by: Agriculture Research Service

Study: Trifolium pratense Raw sequence reads

PRJNA657870 • SRP278075 • All experiments • All runs

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Gene Expression Profiles of a Red Clover CRISPR/Cas9 IFS1 deletion Mutant

Sample: K-01_WT_Nodm

SAMN15856883 • SRS7226127 • All experiments • All runs

Organism: Trifolium pratense

Library:

Name: K-01_WT_Nodm

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq