Normoxia2 - SRA

SRX986252: Normoxia2
1 ILLUMINA (Illumina MiSeq) run: 5.6M spots, 1.7G bases, 832.1Mb downloads

Design: Total RNA from Daphnia was extracted using the mirVanaTM RNA isolation kit (Applied Biosystems) and treated with DNase (Ambion) to remove contaminating genomic DNA. RNA quality was assessed using the Agilent 2100 Bioanalyzer system and samples with a RNA Integrity Number (RIN) greater than 9 were used for mRNA library construction. Briefly, the cDNA libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, USA) following the manufacturer’s protocol. Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and fragmented RNA was subjected to first and second strand cDNA syntheses using random oligonucleotides and SuperScript II, followed by DNA polymerase I and RNase H. After 3’ end adenylation, Illumina PE adapter oligonucleotides were ligated to cDNA. DNA fragments that ligated with adaptor molecules were amplified using the Illumina PCR Primer Cocktail in a 15-cycle PCR reaction. Products were purified and quantified using the AMPure XP and the Agilent Bioanalyzer 2100 systems, respectively. Before sequencing, the libraries were normalized and pooled together in a single lane on an Illumina MiSeq platform. Paired-end reads, each of 150-bp read-length, were sequenced.

Submitted by: The Chinese University of Hong Kong

Study: Daphnia magna Raw sequence reads

PRJNA280795 • SRP057045 • All experiments • All runs

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Transcriptome sequencing of Daphnia Magna

Sample:

SAMN03470177 • SRS901155 • All experiments • All runs

Organism: Daphnia magna

Library:

Name: Normoxia2

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward151 reverse

Runs: 1 run, 5.6M spots, 1.7G bases, 832.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR1964033	5,590,376	1.7G	832.1Mb	2016-04-10

ID:: 1433776

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