Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Endosperms (with the embryo, aleurone, seed coat and pericarp removed) at 5, 10, 15, 20 and 25 DPAs of the T. urartu accession PI 428198 were used for RNA-Seq analysis. Each sample had three independent biological replicates. The total RNA of endosperms was isolated using the standard Trizol reagent method. A library with an insert length of ~350 bp was sequenced using the Illumina HiSeq 2000 (San Diego, CA, USA).