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SRX5100519: UCE target enrichment of Cheilodactylus rubrolabiatus
1 ILLUMINA (Illumina HiSeq 4000) run: 1.4M spots, 266.5M bases, 158.4Mb downloads

Design: Illumina libraries were constructed using the Kapa Hyper Prep Kit (Kapa Biosystems) with dual-indexing barcodes. All reactions followed manufacturer protocols, except reaction sizes were scaled to half the volume indicated by the manufacturer. After library amplification, samples were pooled in equimolar ratios in sets of six to eight samples. Target enrichment of UCE loci was performed on each pool using the MYbaits 0.5k Actinopterygian UCE capture kit (MYcroarray), originally described in Faircloth et al. (2013), following manufacturers protocols. Pools were then amplified and cleaned using 1618 PCR cycles following procedures outlined in Faircloth et al. (2013). All pools were combined in equimolar proportions, and were sequenced either at the University of Georgia Genomics Institute, or the Oklahoma Medical Research Institute, using an Illumina HiSeq or MiSeq Sequencer.
Submitted by: National Museum of Natural History, Smithsonian Institution
Study: A Taxonomic Revision of Cheilodactylidae and Latridae (Centrarchiformes: Cirrhitoidei) Using Morphological and Genomic Characters
show Abstracthide Abstract
Systematic relationships within the family Cheilodactylidae have been uncertain for over a century. This family has a disjunct distribution that has led to regionally-focused studies that have only exacerbated taxonomic confusion. Here we examine systematic relationships within the Cheilodactylidae using ultraconserved genomic elements with near complete taxonomic sampling. We place our results in the context of the Cirrhitoidei – a suborder of five closely related families, and revise the taxonomy of Cheilodactylidae and Latridae. Our results agree with previous findings suggesting that Cheilodactlyidae is polyphyletic, with the type species of the family, Cheilodactylus fasciatus Lacépède, forming a clade with C. pixi Smith that together is more closely related to the Chironemidae than to other cheilodactylids. We also find the majority of cheilodactylids are placed within the Latridae. As a result of our analyses we revise the taxonomy of these two families, name a new genus, and re-elevate Goniistius and Morwong.
Sample: Peter Coulson
SAMN10517325 • SRS4110818 • All experiments • All runs
Library:
Name: Cheilodactylus_rubrolabiatus
Instrument: Illumina HiSeq 4000
Strategy: WGS
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 1.4M spots, 266.5M bases, 158.4Mb
Run# of Spots# of BasesSizePublished
SRR82858821,351,539266.5M158.4Mb2019-05-31

ID:
6877695

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