Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Cells were harvested, immediately frozen in liquid nitrogen, and stored at -80 oC. Total RNA was extracted with phenol and further purified with High Pure RNA Isolation Kit (Roche, Indianapolis,IN). Ribosomal RNA was removed using Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, Inc.) Library was generated by using the TruSeq Stranded mRNA Kit with skipping of poy(A) selection (Illumina, Inc.)