WGS of Lactobacillus murinus, strain NM28_3M-8: murine gut isolate - SRA

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SRX5726460: WGS of Lactobacillus murinus, strain NM28_3M-8: murine gut isolate
1 ILLUMINA (Illumina NovaSeq 6000) run: 6.8M spots, 1.9G bases, 552.3Mb downloads

Design: Sequencing performed by Dr. Brian Yu at the Genomics Platform of the Chan Zuckerberg Biohub. Nextera XT kits were used to make the libraries with CZ Biohubs custom designed adapters - 12 bp index sequences on each side of the library and are dual unique so that each sequence only appears once. The libraries were made in 384 well plates in 4 uL of total volume. Genomic DNA was diluted to 0.18 ng/uL and 0.4 uL was added to the library prep reaction using Mosquito HTS from TTP. 0.5 uL of the library was pooled for sequencing. Sequencing was performed on a NovaSeq S4 flow cell with the run configuration 140 (12) (12) 140, so thats 2x140 bps with dual 12 bp index sequences.

Submitted by: University of Toronto

Study: Genome sequencing and assembly - library of cultured bacterial species from laboratory mouse gut microbiota from the University of Toronto

PRJNA474907 • SRP193474 • All experiments • All runs

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Bank of bacterial mono-isolates from laboratory mouse gut microbiota

Sample:

SAMN10878297 • SRS4664513 • All experiments • All runs

Organism: Ligilactobacillus murinus

Library:

Name: NM28_3M-8

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 6.8M spots, 1.9G bases, 552.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR8946437	6,833,143	1.9G	552.3Mb	2019-04-23

ID:: 7710305

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