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SRX6438777: RNA-sequencing of Drosophila yakuba adult male brain
1 ILLUMINA (NextSeq 500) run: 42.5M spots, 12.7G bases, 4.9Gb downloads

Design: Prior to the dissections, all fly lines were expanded and maintained at 24C under an undisturbed 12:12 light/dark cycle in low density vials with fly food. All individuals were mated and precisely at 4-days-old. Flies were very briefly anesthetized with CO2 and heads were collected with a clean razor blade. Heads were transferred to a glass dissection plate with PBS for brain collection. Dissections were performed within a 3-hour window always at the same circadian zeitgeber time (ZT1-ZT4; in this experiment the light was turned on at 8 am, and the dissections were performed between 9 am and 12 pm within a three-hour interval). Male and female brains of the same line were dissected in parallel the same day at the same time. Dissected brains were immediately transferred into a low retention Eppendorf tube containing 100 mL Trizol (Invitrogen) and kept frozen at -20C until RNA extraction. We collected 3 biological replicates for each genotype by sex combination, and each replicate comprised of a pool of exactly 15 brains.
Submitted by: The Rockefeller University
Study: Transcriptomes of the brain of Drosophila species subgroup
PRJNA553234 • SRP214528 • All experiments • All runs
show Abstracthide Abstract
In this study, we generated and analyzed population scale gene expression data from female and male brains of several strains of Drosophila melanogaster, D. simulans, and D. yakuba to answer questions on gene expression evolution, gene origination, and genome evolution.
Sample: Ta18_male_brain_rep3_L4
SAMN12261918 • SRS5093441 • All experiments • All runs
Organism: Drosophila yakuba
Library:
Name: Ta18_male_brain_rep3_L4
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Runs: 1 run, 42.5M spots, 12.7G bases, 4.9Gb
Run# of Spots# of BasesSizePublished
SRR967843242,473,45412.7G4.9Gb2020-06-10

ID:
8531048

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