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SRX6884315: RNA-seq of Syngnathus scovelli adult female muscle/skin
1 ILLUMINA (Illumina HiSeq 2500) run: 44.7M spots, 13.4G bases, 5.5Gb downloads

Design: Total RNA was sent to the Michigan State University RTSF Genomics Core where libraries were prepared using the TruSeq mRNA Library Prep Kit v2. Libraries were tested for quality control using Caliper GX and qPCR methods. All 30 individuals were barcoded, allowing for each individuals sequence data to be recovered. The 30 libraries were sequenced using two lanes of an Illumina HiSeq 2500 Rapid Run flow cell v1. Base calling was done by Illumina Real Time Analysis (RTA) v1.17.21.3 and output of RTA was demultiplexed and converted to FastQ with Illumina Bcl2fastq v1.8.4
Submitted by: Reed College
Study: Syngnathus scovelli skin and muscle transcriptome
show Abstracthide Abstract
Gulf pipefish (Syngnathus scovelli) exhibit sex-role-reversal with females possessing iridescent bands that are absent in males as well as a deeper body. We took advantage of the fact that males develop the female-typical traits of bands and deeper bodies after exposure to estrogen. We sampled the skin and muscle tissue around the bands in females, females exposed to estrogen, and males exposed to estrogen, as well as skin and muscle tissue in males that lacked a banding pattern. Using the transcripts from the tissues in these four groups we aimed to identify genes associated with maintenance and development of the female secondary sex traits of bands and deeper bodies. We compared expression patterns of control males to those of estrogen-exposed males, estrogen-exposed females, and control females.
Sample:
SAMN12811168 • SRS5417815 • All experiments • All runs
Library:
Name: CF1
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 44.7M spots, 13.4G bases, 5.5Gb
Run# of Spots# of BasesSizePublished
SRR1015893844,733,20613.4G5.5Gb2019-09-21

ID:
9060971

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