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SRX7393580: Pacbio of [Candida] glabrata ATCC2001(CBS138):Pacbio sequencing of Candida grabrata
1 PACBIO_SMRT (PacBio RS II) run: 203,355 spots, 1.3G bases, 909.3Mb downloads

Design: Genomic DNA of strain ATCC2001 (gift of Cecile Fairhead, Pasteur Institute) was made by preparation of spheroplasts, followed by lysis and spooling of high molecular weight genomic DNA after ethanol precipitation. The sequencing library was prepared using DNA Template Prep kit v.2 (3-10kb) following the PacBio shared protocol guidelines for preparing size-selected ~20kb SMARTbell templates. Briefly, 7.5 microgam of genomic DNA was diluted to 150 ul and sheared using Covaris G-tube by centrifugation in an Eppendorf 5424 microcentrifuge at 4600 rpm for 60 seconds. Sheared DNA was then purified using AMPure beads and quality controlled for concentration and size. 5 microgram of sheared DNA was then used for DNA damage repair and end repair. End repaired DNA was purified using AMPure beads and ligated to SMRTbell adapter via blunt end ligation and exonuclease III/VII treatment. The exonuclease treated SMRTbell template was purified again using AMPure beads and quality controlled for yield and library size. The purified SMRTbell library was size selected on Blue Pippin with a size cut off of 5kb. The size selected library was then sequenced on PacBio RS II using PacBio P4/C2 chemistry.
Submitted by: Johns Hopkins Medical Institution
Study: Pacbio sequencing of ATCC2001 (CBS138)
PRJNA596126 • SRP237880 • All experiments • All runs
show Abstracthide Abstract
Pacbio sequencing of Candida glabrata strain ATCC2001 (CBS138) for de novo genome assembly
Sample: CBS138
SAMN13613350 • SRS5841442 • All experiments • All runs
Organism: Nakaseomyces glabratus
Library:
Name: m140326_073259_42159
Instrument: PacBio RS II
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 203,355 spots, 1.3G bases, 909.3Mb
Run# of Spots# of BasesSizePublished
SRR10716749203,3551.3G909.3Mb2019-12-17

ID:
9679567

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