Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: SMGs were harvested, minced and lyzed in RNA lysis buffer and homogenized using Fastprep-24 instrument with lysing matrix D tubes. RNA was purified using RNAqueous-4PCR kit (Ambion, Houston, TX, USA). Total RNA per sample was reverse transcribed, and full-length cDNA was generated using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA). Libraries for Illumina sequencing were prepared using the Nextera XT kit (Illumina), pooled, and analyzed on four lanes of a NextSeq 500 sequencer (Illumina).