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SRX8366270: RNA-seq of Schistocerca nitens: crowded-reared L6 nymph head tissue, biological replicate 3
1 ILLUMINA (Illumina HiSeq 4000) run: 28.8M spots, 8.6G bases, 3Gb downloads

Design: Library preparation was performed using Illuminas TruSeq Stranded Total RNA Library Prep Kit, followed by paired-end sequencing (150 bp) using 8.5 lanes on an Illumina HiSeq4000. Cluster identification, quality prefiltering, base calling and uncertainty assessment happened in real time with Illumina's software (HCS 2.2.68; RTA 1.18.66.3; default parameter settings). Demultiplexing base call files and formatting them into FASTQ files was carried out using Illuminas bcl2fastq script (version 2.17.1.14). All steps were performed at Texas A&Ms AgriLife Research Genomics and Bioinformatics Service.
Submitted by: Texas A&M University
Study: Transcriptome sequencing of four related species of grasshopper in the genus Schistocerca
show Abstracthide Abstract
The main goal of this project is to increase the molecular resources available for the study of density-dependent phenotypic plasticity in locusts, motivated by our long-term interest in the evolution of this extreme form of phenotypic plasticity. Locusts are grasshoppers of which relatively inactive and solitary individuals transform into very active, gregarious individuals in response to an increase in local population density. When high densities persist, locusts will exhibit collective movements that lead to locust plagues. Understanding the molecular basis of this phenomenon has been considered the last frontier in locust research. Over the last few years, we have studied the Central American locust, Schistocerca piceifrons, as a model system. It shows behavioral, morphological, physiological, ecological, and molecular plasticity in response to change in density, similar to the well-studied desert locust S. gregaria. Additionally, S. piceifrons is more closely related to non-swarming grasshoppers than to other locust species within the genus, and these non-swarming relatives also exhibit some degree of density-dependent phenotypic plasticity, reminiscent of a locust. As such, RNA sequencing of two different tissues of S. piceifrons and three of these closely related non-swarming grasshoppers, can help us better understand both the molecular basis of density-dependent phenotypic plasticity itself and of its evolution.
Sample:
SAMN14978110 • SRS6682099 • All experiments • All runs
Library:
Name: SNIT_C3_H_lib
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RT-PCR
Layout: PAIRED
Runs: 1 run, 28.8M spots, 8.6G bases, 3Gb
Run# of Spots# of BasesSizePublished
SRR1181520428,761,1748.6G3Gb2020-05-20

ID:
10881560

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