U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from BioSample

SRX9053860: Target enrichment of UCEs and exons from Myrmelastes leucostigma (Myrmel_leustig_L55190)
1 ILLUMINA (Illumina HiSeq 2000) run: 3.5M spots, 623.4M bases, 386.8Mb downloads

Design: We extracted whole genomic DNA from frozen tissues using standard methods and kits (Qiagen, Valencia, CA). Toepad DNA was extracted using a similar strategy, but in an ancient DNA lab where contamination risks were minimized (detailed protocol available at https://github.com/mgharvey/tyranni). We quantified DNA extracts using a Qubit fluorometer and sent them to Rapid Genomics (Gainesville, FL) for library preparation and sequence capture following the general protocol of Faircloth et al. 2012. This protocol involves fragmenting the genomic DNA, ligating barcoded Illumina-compatible adapters, PCR amplification, probe hybridization, and enrichment of hybridized fragments.
Submitted by: Louisiana State University
Study: Targeted enrichment of ultraconserved elements and exons from suboscine passerines
PRJNA655842 • SRP279468 • All experiments • All runs
show Abstracthide Abstract
The tropics are the source of most biodiversity, yet inadequate sampling obscures answers to fundamental questions about how this diversity evolves. We leverage sampling assembled over decades of fieldwork to study diversification of the largest tropical bird radiation, suboscine passerines, by collecting sequence data using targeted enrichment of 2,389 genomic regions (2,321 UCEs and 96 exons) from 1,940 individuals of 1,287 species.
Sample:
SAMN15864130 • SRS7303766 • All experiments • All runs
Organism: Percnostola leucostigma
Library:
Name: Myrmel_leustig_L55190
Instrument: Illumina HiSeq 2000
Strategy: WGS
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 3.5M spots, 623.4M bases, 386.8Mb
Run# of Spots# of BasesSizePublished
SRR125657293,502,187623.4M386.8Mb2020-12-10

ID:
11763562

Supplemental Content

Related information

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...