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SRX10417962: GSM5202058: B. subtilis 168, exponential phase, rep. #2; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 21.1M spots, 1.6G bases, 596.1Mb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome analysis of miniBacillus PG10 and its parental strain B. subtilis 168
PRJNA716536 • SRP311787 • All experiments • All runs
show Abstracthide Abstract
miniBacillus PG10 lacks ~36% of dispensible genentic material and has been derived from B. subtilis 168 (Reuß et al. 2017; PMID: 27965289). We performed RNA-seq analysis on mid-to-late exponential cultures and stationary phase cultures that were grown in LB medium. We specifically analyzed differential gene expression levels of lipid metabolic genes to compare to the lipidomic profiles of the two strains. Overall design: 12 samples; 3 replicates
Sample: B. subtilis 168, exponential phase, rep. #2
SAMN18437739 • SRS8538897 • All experiments • All runs
Organism: Bacillus subtilis subsp. subtilis str. 168
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Cell were lysed using glassbeads and totRNA was isolated using TRIzol/chloroform rRNA depletion and cDNA library preparation was performed using Zymo-Seq RiboFree Total RNA-Seq Library Kit (Zymo Research, cat. 3003)
Experiment attributes:
GEO Accession: GSM5202058
Links:
Runs: 1 run, 21.1M spots, 1.6G bases, 596.1Mb
Run# of Spots# of BasesSizePublished
SRR1404173121,070,8701.6G596.1Mb2022-05-12

ID:
13736113

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