GSM5202066: PG10, stationary phase, rep. #1; Bacillus subtilis subsp.... - SRA

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SRX10417970: GSM5202066: PG10, stationary phase, rep. #1; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 7.6M spots, 560.8M bases, 216.8Mb downloads

Submitted by: NCBI (GEO)

Study: Transcriptome analysis of miniBacillus PG10 and its parental strain B. subtilis 168

PRJNA716536 • SRP311787 • All experiments • All runs

show Abstracthide Abstract

miniBacillus PG10 lacks ~36% of dispensible genentic material and has been derived from B. subtilis 168 (Reuß et al. 2017; PMID: 27965289). We performed RNA-seq analysis on mid-to-late exponential cultures and stationary phase cultures that were grown in LB medium. We specifically analyzed differential gene expression levels of lipid metabolic genes to compare to the lipidomic profiles of the two strains. Overall design: 12 samples; 3 replicates

Sample: PG10, stationary phase, rep. #1

SAMN18437745 • SRS8538905 • All experiments • All runs

Organism: Bacillus subtilis subsp. subtilis str. 168

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cell were lysed using glassbeads and totRNA was isolated using TRIzol/chloroform rRNA depletion and cDNA library preparation was performed using Zymo-Seq RiboFree Total RNA-Seq Library Kit (Zymo Research, cat. 3003)

Experiment attributes:

GEO Accession: GSM5202066

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 7.6M spots, 560.8M bases, 216.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR14041739	7,577,968	560.8M	216.8Mb	2022-05-12

ID:: 13736121

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