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ERX5111785: Illumina HiSeq 2500 paired end sequencing; Genomic sequencing of Bacillus subtilis isolate NRS6108
1 ILLUMINA (Illumina HiSeq 2500) run: 2.1M spots, 746.8M bases, 459.2Mb downloads

Submitted by: University of Dundee Data Analysis Group
Study: Biofilm hydrophobicity in environmental isolates of Bacillus subtilis
show Abstracthide Abstract
Biofilms are communities of bacteria that are attached to a surface and surrounded by an extracellular matrix. The extracellular matrix protects the community from stressors in the environment, making biofilms robust. The Gram-positive soil bacterium Bacillus subtilis, and in particular the isolate NCIB 3610, is widely used as a model for studying biofilm formation. B. subtilis NCIB 3610 forms colony biofilms that are architecturally complex and highly hydrophobic. The hydrophobicity is linked, in part, to the localisation of the protein BslA at the surface of the biofilm, which provides the community with increased resistance to biocides. As most of our knowledge about B. subtilis biofilm formation comes from one isolate, it is unclear if biofilm hydrophobicity is a widely distributed feature of the species. To address this knowledge gap, we collated a library of B. subtilis soil isolates and acquired their whole genome sequences. We used our new isolates to examine biofilm hydrophobicity and found that, although BslA is encoded and produced by all isolates in our collection, hydrophobicity is not a universal feature of B. subtilis colony biofilms. To test whether the matrix exopolymer poly ?-polyglutamic acid could be masking with hydrophobicity in our hydrophilic isolates, we constructed deletion mutants and found that, contrary to our hypothesis, the presence of poly ?-polyglutamic acid was not the reason behind the observed hydrophilicity. This study highlights the natural variation in the properties of biofilms formed by different isolates and the importance of using more diverse range of isolates as representatives of a species.
Sample: Bacillus subtilis NRS6108
SAMEA8103430 • ERS5790507 • All experiments • All runs
Library:
Name: NRS6108
Instrument: Illumina HiSeq 2500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: Genomic DNA libraries were prepared using Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturer’s protocol with the following modifications: two nanograms of DNA instead of one were used as input, and PCR elongation time was increased to 1 min from 30 seconds.
Runs: 1 run, 2.1M spots, 746.8M bases, 459.2Mb
Run# of Spots# of BasesSizePublished
ERR53227322,074,539746.8M459.2Mb2021-05-11

ID:
14389781

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