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SRX10815625: GSM5285370: n1o2_12_s4; Zea mays; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 32.1M spots, 9.7G bases, 2.8Gb downloads

Submitted by: NCBI (GEO)
Study: Investigation of Gene Network of NKD1, NKD2 and OPAQUE-2 Associated with Maize Endosperm Development
show Abstracthide Abstract
The transcriptomic analysis of nkd1, nkd2 and o2 homozygous mutant set (including 1 WT, 3 single mutants, 3 double mutants and 1 triple mutant) at 8, 12 and 16 DAP revealed temporal regulatory landscape in endosperm grain-filling stage. The data also showed NKD1, NKD2 and O2 interactively regulate gene network overtime in several processes, such as hormone response, cell wall organization, nutrient storage and endoreduplication, etc. Overall design: Transcriptomes of 8, 12 and 16 DAP endosperm from WT (as reference) and homozygous mutant set of nkd1, nkd2 and o2 were profiled and analyzed. All were in a W22 inbred genetic background and 4 biological replicates were conducted for each.
Sample: n1o2_12_s4
SAMN19068260 • SRS8900563 • All experiments • All runs
Organism: Zea mays
Library:
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Dissected samples were immediately frozen in liquid N2 and ground to fine powder. The total mRNA was extracted using conventional SDS/phenol extraction and LiCl/ethanol precipitation protocol Library was prepared with a TruSeq RNA Library Prep Kit (Illumina) and 150-base paired end sequencing using NovaSeq 6000 with the S4 cell platform
Experiment attributes:
GEO Accession: GSM5285370
Links:
Runs: 1 run, 32.1M spots, 9.7G bases, 2.8Gb
Run# of Spots# of BasesSizePublished
SRR1446611832,131,9769.7G2.8Gb2021-12-03

ID:
14354895

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