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SRX14208691: RNA-Seq of the Beaudette FU construct
1 ILLUMINA (Illumina MiSeq) run: 3.8M spots, 1.1G bases, 638.5Mb downloads

Design: Viral RNA library prepped using NEBNext Ultra II RNA Library Prep Kit for Illumina, enriched via hybridization capture using myBaits Hybridization Capture for Targeted NGS with IBV baits and then paired sequenced on a MiSeq
Submitted by: Freie Universitat Berlin
Study: Transcription analysis of avian coronavirus mutants expressing reporter proteins
show Abstracthide Abstract
Infectious bronchitis virus (IBV) is an avian coronavirus that causes infectious bronchitis, an acute and highly contagious respiratory disease of chickens, which is a constant threat and causes significant economic losses worldwide.We have successfully established an infectious clone of IBV strain Beaudette expressing reporter gene. The goal of this study is to analyze if reporter insertion affects genomic RNA and subgenomic RNA synthesis.
Sample:
SAMN26012853 • SRS12030734 • All experiments • All runs
Library:
Name: Beau
Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: VIRAL RNA
Selection: RANDOM PCR
Layout: PAIRED
Runs: 1 run, 3.8M spots, 1.1G bases, 638.5Mb
Run# of Spots# of BasesSizePublished
SRR180566803,789,9281.1G638.5Mb2022-02-22

ID:
20073969

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