Bolinopsis infundibulum pooled larvae PacBio Iso-Seq FLNC - SRA

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SRX14647674: Bolinopsis infundibulum pooled larvae PacBio Iso-Seq FLNC
1 PACBIO_SMRT (Sequel II) run: 2.5M spots, 7.4G bases, 2.7Gb downloads

Design: Total RNA was isolated from approximately 10 mg of 18-hour-post-fertilization B. infundibulum embryos using a Trizol RNA isolation protocol (Rio et al. 2010). The RNA was converted to a PacBio Iso-Seq library and sequenced on one Sequel II SMRT cell at the Brigham Young University DNA Sequencing center. This yielded 2,691,284 CCS Iso-Seq reads, with a mean read length of 3.0 kb and a read N50 of 3.1 kb. Primers and barcodes were removed with Pacific Biosciences lima v2.2.0 (github.com/PacificBiosciences/barcoding), and 2,623,057 full-length non-chimeric (FLNC) reads were generated with Pacific Biosciences isoseq3 v3.4.0 (github.com/PacificBiosciences/IsoSeq).

Submitted by: Monterey Bay Aquarium Research Institute

Study: Bolinopsis microptera Genome sequencing and assembly

PRJNA818620 • SRP366479 • All experiments • All runs

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Genome sequencing and assembly of a single individual of the ctenophore Bolinopsis microptera. Datatypes include PacBio CLR, Chicago, Hi-C. PacBio Iso-Seq reads of pooled larvae are also included.

Sample: pooled 24-hr post-fertilization Bolinopsis infundibulum larvae

SAMN26867237 • SRS12413116 • All experiments • All runs

Organism: Bolinopsis microptera

Library:

Name: DS275

Instrument: Sequel II

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: SINGLE

Runs: 1 run, 2.5M spots, 7.4G bases, 2.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR18516523	2,525,663	7.4G	2.7Gb	2023-02-11

ID:: 20951522

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