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SRX554010: Whole Genome Sequencing of Saccharomyces cerevisiae from New Zealand: Sample 51-TNPLST-4-S-2
1 ILLUMINA (Illumina HiSeq 2000) run: 2.9M spots, 855.9M bases, 570.8Mb downloads

Design: Genomic DNA was sheared into smaller fragments of a desired size by a Covaris E210. The overhangs resulting from fragmentation were converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. After adding an "A" base to the 3' end of the blunt phosphorylated DNA fragments, adapters were ligated to the ends of the DNA fragments. The desired fragments were purified though gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag was added to the adapter at the PCR stage.
Submitted by: The University of Auckland
Study: New Zealand Saccharomyces cerevisiae genome sequencing
PRJNA247448 • SRP042301 • All experiments • All runs
show Abstracthide Abstract
The purpose of this project is to determine the origins, diversity and ancestry of Saccharomyces cerevisiae in New Zealand using whole genome sequencing.
Sample: Saccharomyces cerevisiae TNPLST-4-S-2
SAMN02777156 • SRS621296 • All experiments • All runs
Organism: Saccharomyces cerevisiae
Library:
Name: 51-TNPLST-4-S-2
Instrument: Illumina HiSeq 2000
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Spot descriptor:
1  forward151  reverse

Runs: 1 run, 2.9M spots, 855.9M bases, 570.8Mb
Run# of Spots# of BasesSizePublished
SRR13002022,852,883855.9M570.8Mb2015-07-22

ID:
791354

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