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SRX661401: Roots infected by Verticillium dahliae 7 days Olea europaea
2 ILLUMINA (Illumina HiSeq 1000) runs: 55.1M spots, 11.1G bases, 7.4Gb downloads

Design: RNA was extracted from roots of three plants inoculated with Verticillium dahliae 7 days before. Total RNA was extracted using a Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions. Any DNA contamination was removed by DNaseI treatment on column (Roche, Basel, Switzerland). The RNA-quality tests were performed with the Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) using an RNA 6000 Pico assay kit (Agilent Technologies, Santa Clara, CA, USA). We pooled RNA and made cDNA libraries for sequencing in Illumina HiSeq 1000 sequencer. Two replicates were sequenced on different lines. After trimming and cleaning, we obtained 55.072.107 paired-end reads of 101bp length and 35 Phred quality (11.12 Gb).
Submitted by: Universidad de Jaen
Study: Olea europaea Transcriptome or Gene expression
show Abstracthide Abstract
Control and plants responding to biotic and abiotic stress were sequenced by Illumina HiSeq 1000 paired-end.
Sample:
SAMN02937432 • SRS666334 • All experiments • All runs
Organism: Olea europaea
Library:
Name: Roots VD 7d
Instrument: Illumina HiSeq 1000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Spot descriptor:
forward102  reverse

Runs: 2 runs, 55.1M spots, 11.1G bases, 7.4Gb
Run# of Spots# of BasesSizePublished
SRR152511327,681,4325.6G3.7Gb2014-11-14
SRR152511427,390,6755.5G3.7Gb2014-11-14

ID:
927940

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