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SRX21201023: PacBio HiFi sequencing data from Bombus vosnesenskii: whole male individual (887D)
1 PACBIO_SMRT (Sequel II) run: 1.2M spots, 15.1G bases, 8.4Gb downloads

Design: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (Pacific Biosciences, Menlo Park, CA; Cat. #100-938-900) according to the manufacturer's instructions. HMW gDNA was sheared to a target DNA size distribution between 12 - 20 kb using Diagenodes Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 1.8X of Ampure PB beads (Pacific Biosciences, Menlo Park, CA; Cat. #100-265-900) for the removal of single-strand overhangs at 37C for 15 minutes, followed by further enzymatic steps of DNA damage repair at 37C for 30 minutes, end repair and A-tailing at 20C for 10 minutes and 65C for 30 minutes, and ligation of overhang adapters v3 at 20C for 60 minutes. The SMRTbell library was purified and concentrated with 0.45X Ampure PB beads for size selection with 40% diluted AMPure PB beads to remove short SMRTbell templates, <3 kb. The 12-20 kb average HiFi SMRTbell library was sequenced at UC Davis DNA Technologies Core (Davis, CA) using 0.5 8M SMRT cell, Sequel II sequencing chemistry 2.0, and 30-hour movies each on a PacBio Sequel II sequencer.
Submitted by: UCLA
Study: Bombus vosnesenskii Genome sequencing and assembly
PRJNA998848 • SRP452379 • All experiments • All runs
show Abstracthide Abstract
These data have been produced as part of the CCGP. Reference genomes were generated with PacBio long read data and Dovetail OmniC libraries, and Illumina sequencing at a University of California Sequencing Core. For more information, go to ccgproject.org
Sample: Invertebrate sample from male Bombus vosnesenskii (887D)
SAMN30567649 • SRS18459337 • All experiments • All runs
Organism: Bombus vosnesenskii
Library:
Name: CCGP_gDNA0079
Instrument: Sequel II
Strategy: WGS
Source: GENOMIC
Selection: size fractionation
Layout: SINGLE
Runs: 1 run, 1.2M spots, 15.1G bases, 8.4Gb
Run# of Spots# of BasesSizePublished
SRR254683101,159,34015.1G8.4Gb2023-07-31

ID:
28637345

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