ddradSeq of Lates calcarifer:Offspring 170 - SRA

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SRX17905860: ddradSeq of Lates calcarifer:Offspring 170
1 ILLUMINA (NextSeq 500) run: 2.2M spots, 640.6M bases, 249.7Mb downloads

Design: In brief, 500 ng of DNA from each sample was double-digested with 20 units of EcoRI-HF and NIaIII (New England Biolabs, USA) for 30 min at 37C. Digested DNA fragments were examined by electrophoresis and then ligated with barcoded adaptors of P1 that contained a matching sticky-end and a MID (a short sequence that will uniquely identify the individuals) and P2.

Submitted by: James Cook University

Study: Mapping of a Major QTL for Increased Robustness and Detection of Genome Assembly Errors in Asian Seabass (Lates calcarifer)

PRJNA890911 • SRP402860 • All experiments • All runs

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A farm-based challenges were conducted by exposing Asian seabass juveniles to complex natural environmental conditions. In one of these challenges, a total of 1,250 fish classified as either sensitive or robust individuals were collected during the 28 day observation period. A high-resolution linkage map with 3,089 SNPs was constructed for Asian seabass using the double digest Restriction-site Associated DNA (ddRAD) technology and a search for Quantitative Trait Locus (QTL) associated with robustness was performed.

Sample:

SAMN31303288 • SRS15422571 • All experiments • All runs

Organism: Lates calcarifer

Library:

Name: F2P3_DS_D6

Instrument: NextSeq 500

Strategy: RAD-Seq

Source: GENOMIC

Selection: size fractionation

Layout: PAIRED

Runs: 1 run, 2.2M spots, 640.6M bases, 249.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR21920582	2,167,349	640.6M	249.7Mb	2023-06-21

ID:: 24844262

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