SRX18280311: long-read sequencing of the tprK gene
1 PACBIO_SMRT (Sequel) run: 1,377 spots, 2.3M bases, 651,480b downloads
Design: The DNA extracting from 14 primary syphilis samples and 14 secondary syphilis samples was directly used for PCR amplification of tprK. PCR was conducted with tprK-specific primers appended to 16-bp PacBio barcodes under the following conditions: 98C for 2 minutes followed by 35 cycles of 98C for 10 seconds, 62C for 15 seconds and 68C for 2 minutes with a final elongation at 68C for 5 minutes. The resulting tprK amplicon was purified using 0.6x volumes of AMPure XP beads. barcodes (Table S1) under the following conditions: 98C for 2 minutes followed by 35 cycles of 98C for 10 seconds, 62C for 15 seconds and 68C for 2 minutes with a final elongation at 68C for 5 minutes. The resulting tprK amplicon was purified using 0.19x volumes of AMPure XP beads ((Beckman Coulter, Inc., CA, USA).
Submitted by: Zhongshan Hospital, Medical College of Xiamen University, Xiamen, China
Study:
PacBio long-read sequencing of the tprK geneshow Abstracthide AbstractEstimation of full-length tprK varaints diversity in Treponema pallidum subsp. pallidum
Library:
Name: X14
Instrument: Sequel
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: SINGLE
Runs:
1 run, 1,377 spots, 2.3M bases, 651,480b