GSM7000668: Dryas_iulia_F1; Dryas iulia; OTHER - SRA

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SRX19205922: GSM7000668: Dryas_iulia_F1; Dryas iulia; OTHER
1 ILLUMINA (NextSeq 500) run: 27.1M spots, 2.2G bases, 807.4Mb downloads

External Id: GSM7000668_r1

Submitted by: Danko and Lis labs, Molecular Biology and Genetics, Cornell University

Study: Pol II pausing is a milestone on the road to complex animals

PRJNA929029 • SRP419683 • All experiments • All runs

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PRO-seq experiments in multiple organisms. Also, PRO-seq experiment of Nelfb and Nelfe degron mESC in serum/LIF with or without dTAG-13. Overall design: We used Precision Run-On and Sequencing (PRO-seq) to study transcription in 20 extant organisms that represent very diverse branches on the tree of life, including multiple species near the base of the Metazoa phylogeny and the transition between plants and animals. We also used PRO-seq to study mESCs after degrading NELF-B or NELF-E.

Sample: Dryas_iulia_F1

SAMN32949461 • SRS16614682 • All experiments • All runs

Organism: Dryas iulia

Library:

Name: GSM7000668

Instrument: NextSeq 500

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Construction protocol: RNA extraction, run-on, and library preparation were executed according to “Mahat, D. B. et al. Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on (PRO-seq). Nat. Protoc. 11, 1455–1476 (2016).” (PMID: 27442863). Library prep was performed using Illumina custom adapters: Reverse 3' RNA adapter (/5Phos/NNNNNNGAUCGUCGGACUGUAGAACUCUGAAC/3InvdT) and Reverse 5' RNA Adapter (5'-CCUUGGCACCCGAGAAUUCCA-3').

Runs: 1 run, 27.1M spots, 2.2G bases, 807.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR23260640	27,108,178	2.2G	807.4Mb	2024-02-05

ID:: 26407917

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