Name: GSM7064221
Instrument: Illumina NovaSeq 6000
Strategy: ATAC-seq
Source: GENOMIC SINGLE CELL
Selection: other
Layout: PAIRED
Construction protocol: Single-nuclei cDNA library preparation and sequencing were performed following manufacturer's protocols (https://www.10xgenomics.com). Single-nuclei suspension was loaded on a Chromium controller to obtain single cell GEMS (Gel Beads-In-Emulsions) for the reaction. The snATAC-seq library was prepared with Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1 (10x Genomics). Human retina samples were collected by the Utah Lions Eye Bank within 6 hours postmortem and were confirmed to be free of drusen, atrophy, or other disease pathology. The fovea and macula samples were collected separately, using a 4 mm and 6 mm disposable biopsy punch, respectively, and were flash-frozen in liquid nitrogen. All samples were then stored at −80 °C before nuclei isolation. Nuclei were isolated by pre-chilled fresh-made RNase-free lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.02% NP40). The frozen tissue was resuspended and triturated to break the tissue structure in lysis buffer and homogenized with a Wheaton™ Dounce Tissue Grinder. Single-nuclei cDNA library preparation and sequencing were performed following manufacturer's protocols (https://www.10xgenomics.com). Single-nuclei suspension was loaded on a Chromium controller to obtain single cell GEMS (Gel Beads-In-Emulsions) for the reaction. The snRNA-seq library was prepared with Chromium Next GEM Single Cell 3' Kit v3.1 (10x Genomics). The snATAC-seq library was prepared with Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1 (10x Genomics). The library was then sequenced on Illumina Novaseq 6000