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SRX19582365: GSM7086186: Midgut, 2 hours after feeding, replicate 2; Schistocerca gregaria; RNA-Seq
8 ILLUMINA (NextSeq 500) runs: 61.7M spots, 4.7G bases, 1.7Gb downloads

External Id: GSM7086186_r1
Submitted by: Ghent University
Study: Differential midgut transcriptomics comparing nutritional status reveals a crucial role for V-ATPase subunit a and Niemann-pick 1b protein in the digestive physiology of the desert locust.
PRJNA941911 • SRP425996 • All experiments • All runs
show Abstracthide Abstract
The main objective of this study was to analyze the general midgut transcript profile of Schiustocerca gregaria as well as changes in midgut gene expression during different stages of feeding. We compared three different time points of the digestive process: no active digestion (24 h after feeding), the initial stages of feeding (10 min after feeding) and active digestion in the midgut (2 h after feeding). Overall design: Samples are RNA extracts of the desert locust midgut. We compared 3 timepoints. Each timepoint consists of 6 biological replicates.
Sample: Midgut, 2 hours after feeding, replicate 2
SAMN33619992 • SRS16965264 • All experiments • All runs
Organism: Schistocerca gregaria
Library:
Name: GSM7086186
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted using the RNeasy lipid tissue extraction kit (Qiagen) according to the manufacturer's protocol. An additional DNaseI treatment (Qiagen) was performed to remove potential genomic DNA contamination. For Illumina® deep sequencing, mRNA was first purified from the total RNA mixture in every sample. From each sample, 1 μg of RNA was used to perform an Illumina® sequencing library preparation using the TruSeq® Stranded mRNA Library prep kit (Illumina®) according to the manufacturer's protocol.
Runs: 8 runs, 61.7M spots, 4.7G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR237214158,375,487632.2M231.5Mb2023-09-13
SRR237214168,321,460628.1M229.9Mb2023-09-13
SRR237214188,378,522632.4M235.7Mb2023-09-13
SRR237214437,092,680535.3M203Mb2023-09-13
SRR237214446,994,156527.9M200.3Mb2023-09-13
SRR237214608,298,339626.3M234.4Mb2023-09-13
SRR237214617,182,255542.1M201.6Mb2023-09-13
SRR237214627,070,660533.7M198.5Mb2023-09-13

ID:
26875565

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