Name: GSM7086182
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted using the RNeasy lipid tissue extraction kit (Qiagen) according to the manufacturer's protocol. An additional DNaseI treatment (Qiagen) was performed to remove potential genomic DNA contamination. For Illumina® deep sequencing, mRNA was first purified from the total RNA mixture in every sample. From each sample, 1 μg of RNA was used to perform an Illumina® sequencing library preparation using the TruSeq® Stranded mRNA Library prep kit (Illumina®) according to the manufacturer's protocol.