SRA

Halimocnemis mollissima Bunge displays two photosynthetic mechanisms in their life cycle. As such, they provide an excellent model for studying the molecular regulatory mechanisms underlying the coordination of roots and modified shoots during the C3 to C4 conversion while eliminating phylogenetic noises that hindered earlier models. Unlike the C3 pathway in cotyledons, which coincides with moderate temperature, the C4 pathway in this plant, which belongs to the NAD-ME subtype of C4 photosynthesis, outperforms at higher light and temperature conditions concurrent with the formation of the first leaf (FL) stage onwards.To unravel the signaling pathways associated with the C3 to C4 transition in H. mollissima Bunge, we used RNA sequencing to generate transcriptome profiles of hypocotyls before and after forming the C4 FLs alongside the FLs. Genes associated with several phytohormone metabolisms, TFs, and signal peptides were differentially expressed in hypocotyls before and after forming C4 FLs.