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SRX19935630: RNA-Seq of green turtle's blood
1 ILLUMINA (Illumina NovaSeq 6000) run: 22.4M spots, 6.7G bases, 2.1Gb downloads

Design: 1) Enrichment of eukaryotic mRNA with magnetic beads with Oligo (dT);(2) Random interruption of mRNA by addition of Fragmentation Buffer;(3) Synthesis of the first cDNA strand and the second strand using mRNA as a template and cDNA purification;(4) The purified double-stranded cDNA is then end-repaired, A-tailed and ligated to sequencing junctions, followed by fragment size selection using AMPure XP beads;(5) Final enrichment by PCR to obtain cDNA libraries
Submitted by: Institute of Zoology, Guangdong Academy of Sciences
Study: Transcriptome of green sea turtle whole blood
show Abstracthide Abstract
To study the immunity of green sea turtles
Sample:
SAMN34146937 • SRS17285402 • All experiments • All runs
Organism: Chelonia mydas
Library:
Name: B1
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: Oligo-dT
Layout: PAIRED
Runs: 1 run, 22.4M spots, 6.7G bases, 2.1Gb
Run# of Spots# of BasesSizePublished
SRR2413701222,397,7236.7G2.1Gb2024-05-07

ID:
27311158

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