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SRX20107038: GSM7230409: 24h post-infection (hpi) uninduced [UI σ28/σ54 KD 2]; Chlamydia trachomatis 434/Bu; RNA-Seq
2 ILLUMINA (Illumina NovaSeq 6000) runs: 11.7M spots, 2.3G bases, 777.5Mb downloads

External Id: GSM7230409_r1
Submitted by: Ouellette, Pathology and Microbiology, University of Nebraska Medical Center
Study: Identification of the alternative sigma factor regulons of Chlamydia trachomatis using multiplexed CRISPR interference
PRJNA962055 • SRP434626 • All experiments • All runs
show Abstracthide Abstract
RNAseq analysis was performed for C. trachomatis serovar L2 after knocking down or overexpressing different sigma factors. Overall design: RNA was collected from C. trachomatis L2 infected cells following 14 hours of sigma factor knockdown or 4 hours of sigma factor overexpression. Each strain was tested in triplicate, resulting in 48 total samples. Induced knockdown samples were compared to their respective uninduced samples as well as an induced non-targeting knockdown sample. Overexpression samples were compared to their respective uninduced samples.
Sample: 24h post-infection (hpi) uninduced [UI σ28/σ54 KD 2]
SAMN34380316 • SRS17437945 • All experiments • All runs
Organism: Chlamydia trachomatis L2/434/Bu
Library:
Name: GSM7230409
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was collected from C. trachomatis infected cell cultures using Trizol. Total RNA was DNased then further purified to remove eukaryotic mRNA and both prokaryotic and eukaryotic rRNA using Oligotex, MicrobEnrich, MicrobExpress, and Ribozero. Illumina Ribo-Zero plus Microbiome
Runs: 2 runs, 11.7M spots, 2.3G bases, 777.5Mb
Run# of Spots# of BasesSizePublished
SRR243108146,037,8041.2G397.4Mb2023-09-05
SRR243108155,683,4311.1G380.1Mb2023-09-05

ID:
27516102

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